Fig. 6

Inhibiting adipose ILC1s accumulation attenuates adipose fibrosis. Rag1^−/− mice were fed with normal diet or HFD, while mice in the HFD group were randomized into two groups of HFD anti-IL-12 mAb group and HFD control IgG group and continued with HFD feeding for another 4 weeks. HFD anti-IL-12 mAb group received rat anti-murine anti-IL-12p35 mAb administration via intraperitoneal injection at 250 μg per mouse every 3 days for 4 weeks; HFD control IgG group received the same dose of control IgG. a Representative plots indicate adipose ILC1s in the VAT of mice with different treatment. b Absolute density (cells mg⁻¹) of adipose ILC1s in VAT. c Absolute density of CD11c⁺ macrophages (cells mg⁻¹) in VAT. d Body weight. e Fasting blood glucose. f Glucose tolerance test. g Representative Masson’s trichrome C and Sirius red staining in VAT of mice with different treatment (scale bar: 100 μm). Arrows indicate positive staining areas. h Expression levels of fibrosis-related genes, assessed by q-PCR. i Representative microscopic images with H&E staining in the liver (scale bar: 20 μm). j, k Hepatic triglyceride levels and serum free fatty acid (FFA) levels. ^**P < 0.01 vs. CHOW group; ^#P < 0.05 vs. HFD+control IgG group; ^##P < 0.01 vs. HFD+control IgG group (two-way ANOVA tests followed by Bonferroni post hoc test). The data are representative of two independent experiments, with n = 5 mice per group. Error bars indicate s.d.