Fig. 1
KLF-1 mediates the longevity of mitochondrial isp-1;ctb-1 mutant. a Lifespan curves of isp-1(qm150);ctb-1(qm189) mutant (left) and wild-type animals (middle) grown on control (empty vector, EV) or klf-1 RNAi plates. Right panel shows lifespan curve of klf-1(tm1110) mutant animals. b isp-1(qm150);ctb-1(qm189) mutants (left) and wild type animals (right) were grown on control (EV) RNAi plates until L4 developmental stage and then transferred to klf-1 RNAi. c Lifespan curve of isp-1(qm150);ctb-1(qm189) mutant (left) and wild-type animals (right) grown on klf-1 RNAi plates until L4 developmental stage and then transferred to control (EV) RNAi. d klf-1 expression level was assessed by qPCR at the L4 larval stage, the first (D1) and the fifth day (D5) of adulthood, in the wild-type and isp-1(qm150);ctb-1(qm189) mutants. Data are presented as mean ± SEM. **p < 0.01, ***p < 0.001, one-way ANOVA with Tukey post hoc test. n = 5 samples per condition. e Fluorescent images of gfp expression under klf-1 promoter in wild type and isp-1(qm150);ctb-1(qm189) at the first day of adulthood. Scale bar 100 µm
