Fig. 4
KLF-1 mediates expression of cytochrome P450 oxidases. a log2 fold-change in gene expression in isp-1(qm150);ctb-1(qm189) mutant animals compared with N2 wild type, grown either on control (EV) or klf-1 RNAi from L4 larval stage. Microarray analysis was performed at the fifth day of adulthood. b The expression of cytochrome P450 oxidases from (a) was confirmed by qPCR. n = 4 independent samples per condition. Animals were analysed at the first (D1) or the fifth (D5) day of adulthood and grown on control (EV) and klf-1 RNAi whole life. c CHIP-qPCR analysis of WT and isp-1;ctb-1 for cyp-13a11 promoter region. n = 2 independent replicates. d Levels of Cyp2C70 and Cyp3A13 were assayed with qPCR in Hepa1-6 cells with or without antimycin A (AA) treatment as indicated. Cells were exposed to either Klf4, Klf5 or control siRNA. Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA with Tukey post hoc test. n = 4 independent samples per condition
