Table 2 Production of limonene in semi-batch conditions
From: Orthogonal monoterpenoid biosynthesis in yeast constructed on an isomeric substrate
Protein expressed | Limonene (mg L−1) in MIC2 | Nerol (mg L−1) in MIC2 | Limonene (mg L−1) in MIC3 | Nerol (mg L−1) in MIC3 |
|---|---|---|---|---|
Erg20p(N127W) + ClLimS wt | 23.31 ± 5.97 | – | 41.49 ± 3.95 | – |
Erg20p(N127W) + ClLimS(H570Y) | 27.51 ± 5.04 | – | 86.30 ± 10.76 | – |
Erg20p(N127W)-SlNPPS1 + ClLimS wt | 62.47 ± 15.32 | 0.542 | 71.84 ± 13.98 | 0.042 |
Erg20p(N127W)-SlNPPS1 + ClLimS(H570Y) | 126.19 ± 18.97 | 0.023 | 167.38 ± 20.88 | 0.015 |