Fig. 6
From: Activation of DR3 signaling causes loss of ILC3s and exacerbates intestinal inflammation
DR3-Fc treatment ameliorates α-CD40-induced colitis. Female littermate Rag1–/– mice were injected with 50 µg of α-CD40. a Expression of Tnfsf15 mRNA in large intestinal LPLs was analyzed by real-time RT-PCR 48 h after treatment. b–j 200 µg of DR3-Fc or control IgG or PBS were i.p. administered daily from day 0. c–g, i, j Mice were killed for analysis on day 3. b Percentages of weight change are shown. Representative picture of large intestine (c) and lengths of colons (d) from indicated groups are shown. e, h, i Large intestinal LPLs were isolated, and the expression of Lin, RORγt, CD11b, and Ly6G was analyzed by flow cytometry. e Percentages of neutrophils (CD11b+Ly6G+) in live cells are shown. i Percentages of ILC3s (Lin–RORγt+) gated on lymphocytes are shown. j The total numbers of ILC3s were calculated and are shown. h Mice were killed for analysis 24 h later after α-CD40 injection, and large intestinal LPLs were isolated. Expression of Lin, RORγt, and GM-CSF was analyzed by flow cytmetry. Percentages of GM-CSF+ cells in ILC3s are shown. Statistical analysis was performed with Student’s t test. f Paraffin-embedded colon sections were stained with hematoxylin and eosin (H&E). Scale bar is 100 µm. g Histological scores were evaluated and shown. The data are means ±;SEM. a–j The data are representative of at least two independent experiments. Source data are provided as a Source Data File
