Fig. 3

PSMA8 is required for proteasome assembly and protein degradation. a Western blotting results showing the elevated ubiquitination level in Psma8^−/− testes at the age of PD21. b PSMA8 deletion destabilized the proteasomes, as shown by α sub immunofluorescent staining in sections of PD25 testes. P pachynema. Scale bars, 25 μm. c Western blotting results showing the instability of proteasomes and failure of their ability to degrade prophase I proteins (RAD51 and RPA1) in Psma8^−/− testes at the age of PD42. The band intensity from three independent western blottings are quantified and the relative values are shown underneath each band. d Immunofluorescent staining of PSMA7 in testes sections derived from WT and Psma8^−/− males at PD42. Scale bars, 25 μm