Fig. 1

Olfactory bulb architecture is preserved in EPL-IN Vgat knockouts. a Illustration of OB connectivity (left). OB coronal section (right) with TdTomato positive EPL-INs (magenta) and anti-Tbx21 positive TC/MC (green) in the EPL and MCL layers. b Illustration depicting normal Vgat protein function for neurotransmitter vesicle loading (Top) and after excision of the Vgat^flox/flox allele (Bottom). c Top: In situ hybridization against VGAT mRNA between control and EPL Vgat knockout. Bottom: Thresholded black/white image from top images. d Quantification of Vgat positive area within the EPL. All areas normalized to the average control area. Two-tailed t-test, **p < 0.01. e Frequency measurements of sIPSC onto MCs between Control vs EPL Vgat KO. Two-tailed t-test. f Frequency measurements of sIPSC onto TCs between Control vs EPL Vgat KO, Two-tailed t-test, *p < 0.05. g Antibody staining to compare control and EPL Vgat knockout animals for Tbx21 positive excitatory neurons and calretinin-positive inhibitory neurons, h parvalbumin positive EPL-INs, and i tyrosine hydroxylase (TH) positive SACs. j Cell density comparisons (two-tailed t-test) between control and experimental animals for MCs, TCs, GCs, EPL-INs, and SACs. Cell densities normalized to controls. Averages ± SEM. All scale bars 100 μm