Fig. 4
From: Target specific functions of EPL interneurons in olfactory circuits
Two-photon imaging of odor responses. a Thy1-GCaMP6F transgenic mice showing GCaMP6F positive MCs (hollow triangle) and TCs (solid triangle) (left) as identified by anti-TBX21 labeling (middle, right). b Thy1-GCaMP6F allele in control and EPL-IN Vgat knockout animals. c In vivo 2-photon imaging in awake, head-fixed rodents. d Example cortical window for 2-photon experiment (left). Example GCaMP imaging of different depths for TCs (middle) and MCs (right). Scale bars, 100 μm. e Example calcium traces from 18 TCs, with odor stimulation periods represented as colored bars. Scale bars represent 200% df/f. The respiration measured as temperature modulation is shown in the bottom trace. Insert shows a small segment of the respiration during one trial. f Average breathing rate (Hz) as a function of time in the experiment for all animals
