Fig. 2
From: Modification of messenger RNA by 2′-O-methylation regulates gene expression in vivo
Pxdn mRNA modification by Nm requires box C/D snoRNAs. 293T cell lines with CRISPR/Cas9 knockout (KO) for U32A (n = 3), U51 (n = 3), or U32A + U51 (n = 4). Each KO cell line is an independent biological sample, analyzed in two independent experiments. Mean and SEM error bars, *p < 0.05 vs. 293T, by one-way ANOVA. a Pxdn mRNA from snoRNA KO cells showed loss of Nm (*p = 0.0086). b snoRNA KO cells showed reduced Pxdn mRNA (*p = 0.0128). c Peroxidase activity in cells was measured using Amplex Red and normalized to WT (*p = 0.039)
