Fig. 6

Immune response of the public αβTCR transfectants to the drug antigen and HLA. a Parental 5KC or 5KC-TCR cells were co-cultured with C1R or C1R-B*15:02 cells and stimulated with CBZ (25 μg/ml) or solvent control. The immune response of the 5KC-TCR transfectants was determined by the IL2 ELISPOT assay. The results are representative of three independent experiments, and expressed as mean ± s.e.m. with each dot representing the data of one sample. Statistical analysis was performed using an unpaired, two-tailed Student’s t test. b A representative IL2 ELISPOT data set of Fig. 6a is shown. c Response of 5KC-TCR and C1R-B*15:02 co-cultures to different antiepileptic drugs at physiological concentrations (i.e., CBZ: 25 μg ml⁻¹; CBZ-epoxide: 10 μg m⁻¹; oxcarbazepine: 25 μg ml⁻¹; gabapentin: 6 μg ml⁻¹; phenobarbital: 15 μg ml⁻¹; valproic acid: 100 μg ml⁻¹). The results are representative of three independent experiments, and expressed as mean ± s.e.m. with each dot representing the data of one sample. Statistical analysis was performed using an unpaired, two-tailed Student’s t test. d Responses of 5KC-TCR and C1R-B*15:02 co-cultures to CBZ or CBZ-epoxide at serial concentrations (in micromoles). The data are shown as mean ± s.e.m. of triplicate measurements for each condition and each dot representing the data of one sample. The data were analyzed using an unpaired, two-tailed Student’s t test. “5KC-TCR” represents 5KC cells expressing the public αβTCR composed of the paired TCRα CDR3 “VFDNTDKLI” and TCRβ CDR3 “ASSLAGELF” clonotypes. CBZ carbamazepine. **P < 0.01