Table 1 Preferential TCRβ CDR3 clonotypes in blister cells from patients with CBZ-SJS/TEN

From: Identification of drug-specific public TCR driving severe cutaneous adverse reactions

Case no.

Culprit drug

Symptom

 

CDR3 clonotypes

TRBV

TRBJ

Frequency (%)

Specific CDR3 reads/total reads

Frequency (%) in PBMC of healthy donorsa

Case 1

CBZ

SJS/TEN

1

ASSLSDTIY

12–4

1–3

24.45

182480/746406

0

2

ASSLGGAPY

12–4

2–7

8.41

62776/746406

0

3

ASSYNPGTGTEEYEQY

6–1

2–7

6.50

48516/746406

0

4

ASTHTGELF

12–4

2–2

4.60

34325/746406

0

5

ASSLAGELF

12–4

2–2

3.73

27829/746406

<0.001

6

ASSPRLAGSTDTQY

27

2–3

2.11

15748/746406

<0.001

Case 2

CBZ

SJS

1

ASSLAGELF

12–4

2–2

19.39

153620/792164

<0.001

2

ASSLSGYEQY

27

2–7

4.01

31781/792164

0.0026

3

ASSSAGEVF

12–4

2–1

2.27

17990/792164

0

Case 3

CBZ

SJS

1

ASSLAGELF

12–4

2–2

6.64

5411/81449

<0.001

2

ATSGPNQETQY

24–1

2–5

2.08

1697/81449

0

Case 4

CBZ

TEN

1

ASSLAGELF

12–4

2–2

42.85

31148/72692

<0.001

2

ASSYRDGYEQY

6–5

2–7

2.73

1986/72692

<0.001

3

ASSRRAVVGSYNEQF

7–2

2–1

2.62

1905/72692

0

4

ATHGTGYLEQY

25–1

2–7

2.15

1565/72692

0

Case 5

CBZ

SJS/TEN

1

ASSLAGELF

12–4

2–2

17.41

143248/822649

<0.001

2

ASSSRLAGGTDTQY

27

2–3

12.11

99586/822649

0.0025

Case 6

CBZ

SJS/TEN

1

ASSPSDRSSYEQY

18

2–7

4.69

57694/1229012

0

2

ASSSLTSSWVEQF

28

2–1

3.81

46784/1229012

0

3

ASTSGPNEQF

12–4

2–1

2.99

36759/1229012

<0.001

4

ASSYSSTDTQY

6–5

2–3

2.80

34429/1229012

0.0029

5

ASSQYRYNEQF

14

2–1

2.60

32006/1229012

0

6

ASSFAGELF

12–4

2–2

2.22

27338/1229012

<0.001

7

ASSLAGELF

12–4

2–2

1.08

13273/1229012

<0.001

Case 7

CBZ

TEN

1

ASSWDPTIY

12–3

1–3

10.21

81449/798017

0

2

ASSLAGELF

12–4

2–2

4.00

31881/798017

<0.001

3

ASIDGSSLNEQF

27

2–1

3.57

28498/798017

0

4

ASSLSGYEQY

27

2–7

2.90

23122/798017

0.0026

5

ASSYSDTIY

6–6

1–3

2.50

19948/798017

0

  1. CDR3 third complementarity-determining region, PBMC peripheral blood mononuclear cell, SJS Stevens–Johnson syndrome, TEN toxic epidermal necrolysis, TRBJ T-cell receptor β joining, TRBV T-cell receptor β variable
  2. aThe mean frequencies of the corresponding CDR3 clonotypes in PBMC of healthy donors (n = 44)
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