Fig. 5
PVH→LSv projections regulate feeding. Sim1-Cre and Sim1-Cre::Vglut2flox/flox male mice with AAV-Flex-ChR2-eGFP delivery to the PVH and fiber optic implantation targeting LSv were used for feeding assays. a Measurements of food intake over 15 mins after either 20 hr (n = 6–15, OFF vs 5 HZ,100 ms p = 0.0306; 5 HZ,10 ms vs 5 HZ,100 ms p = 0.0108), 12 hr (n = 6, OFF or 5 HZ,100 ms vs 5 HZ,100 ms, p < 0.0001) or 6 hr (n = 6, p = 0.0002) fasting with no photostimulatoin (OFF), long pulse (100 ms/5 Hz, 5 mW/mm2), or short pulse photostimulation (10 ms/5 Hz, 5 mW/mm2). b Measurements of feeding time with alternating segments of laser off (OFF) and on (ON, 100 ms/5 Hz, 5 mW/mm2) at 1 min each (n = 5–8/group, p < 0.001, p = 0.0277, or p = 0.0181; also online Movie 4, Source data are provided as a Source Data file). c–e A representative image showing AAV-Flex-ChR2-eGFP expression and optic fiber implantation trace in the LSv of Vgat-Cre animals after stereotaxic delivery of the vector to LSv c, feeding was measured for 15 mins after 12 hr fasting with no light (OFF), long pulse photostimulation (ON) (d, n = 5, p = 0.0049), or measured with alternating segments of laser off (OFF) and the long pulse (ON) at 1 min each (n = 5) (e, also online Movie 5, Source data are provided as a Source Data file). f Well-fed mice with cannulation targeting LSv were measured for feeding 20 mins after infusion of AP5+DNQX or saline (n = 5, p < 0.0001, also online Movie 6). g–h Well-fed Sim1-Cre mice with AAV-Flex-ArchT3.0-eGFP bilateral delivery to PVH (g, left panel, arrows) and optic fiber implantation targeting LSv harboring GFP-expressing fibers (g, right panel, arrows) were measured for feeding in 2 hrs with yellow laser off (OFF) and following a long pulse on (ON, n = 5, p = 0.0268) h. *p < 0.05, ***p <0.001, student’s t tests for comparison between two groups (b, d, f, and h) or ANOVA tests for comparison among three groups a. Scale bar = 100 µm. PVH: paraventricular hypothalamus; LV: lateral ventricle; 3 V: the third ventricle; LSv: ventral part of lateral septum; aca: anterior commissure area; AcbSh: accumbens shell. Source Data
