Fig. 6
Place aversion by photostimulation of PVH→LSv terminals antagonizes hunger-driven feeding. Male mice with AAV-Flex-ChR2-eGFP delivery to PVH and optic fiber implantation targeting LSv were fasted 12 hr before RTPP tests, in which chow diet pellets were placed in the corner of the side paired with photostimulation PVH→LSv fibers a–d. Representative movement traces were shown for Sim1-Cre control mice with AAV-Flex-GFP injection a, Sim1-Cre mice with short pulse (ON,10 ms, 5 Hz, 5 mW/mm2, b, long pulse photostimulation (ON,100 ms, 5 Hz, 5 mW/mm2, c) and for Sim1-Cre::Vglut2flox/flox mice with long pulse photostimulation (ON, d). e–g Time spent in zones in Sim1-Cre mice with AAV-Flex-GFP delivery (e, n = 5; On+Food zone vs On+Non-food zone, p = 0.0126; On+Food zone vs OFF zone, p = 0.0016) or with AAV-Flex-ChR2-eGFP delivery, and Sim1-Cre::Vglut2flox/flox mice with AAV-Flex-ChR2-eGFP delivery (f, n = 7; On+Food zone vs On+Non-food zone or OFF zone, p < 0.0001). g Food intake was measured during a 15 min testing period in d–f, n = 5–7/group (Sim1-Cre mice 5 HZ,10 ms vs 5 HZ,100 ms, p = 0.0130; all other comparisons, p < 0.0001). *p < 0.05, **p < 0.01 and ***p < 0.001, one-way (e, f, and g) or two-way (e ANOVA tests
