Fig. 7
In vivo Ca2+ fiber photometry measurements of PVH and LSv neuron activity during stress and feeding. Sim1-Cre a, c, e, and g and Vgat-Cre male mice (b, d, f, and h) with AAV-Flex-GCaMP6m delivery to the PVH (a, c, e, and g) or LSv (b, d, f, and h) were implanted with fiberoptics targeting the PVH or LSv for fiber photometry monitoring the in vivo activity of PVH and LSv neurons in freely moving mice. A loud sound (a and b), a brief light exposure in dark (c and d), water spray toward head (e and f) were associated with rapid activation of PVH (a, c, e, and g) and LSv (b, d, f, and h) neurons. In contrast, the activity of PVH g and LSv h neurons was highly associated with eating bouts; and i summary data showing comparison in activity changes indicated by averaged means in Ca2+ imaging between periods with feeding bouts and the testing period in the groups indicated. PVH-GFP: recording from PVH neurons expressing GFP mediated by Sim1-Cre; PVH-GCaMP6m: recording from PVH neurons expressing GCaMP6m mediated by Sim1-Cre, and LSv-GCaMP: recording from LSv neurons expressing GCaMP6m mediated by Vgat-Cre (n = 8–10 each group). ***p < 0.001, two-way ANOVA tests, eating versus non-eating bouts. The red lines in a–f represent averaged means of traces from the indicated individual stressing events
