Fig. 8 | Nature Communications

Fig. 8

From: Identification of a neurocircuit underlying regulation of feeding by stress-related emotional responses

Fig. 8

Tracing between PVH and LSv. a–h Retrograde tracing from the LSv to PVH. a Diagram showing delivery of retrograde AAVrg-Cre-Venus to the LSv of adult wild-type male mice and examination of traced upstream neurons including the PVH. Confirmation of vector expression in the LSv b, and the traced neurons in brain sections with various Bregma levels from rostral to caudal PVH c–h. i–l Anterograde tracing from the PVH to LSv. i Diagram showing delivery of anterograde AAV1-Cre-GFP to the PVH of adult wild-type mice and of Cre-dependent AAV-DIO-mCherry vectors to the LSv for examination of traced downstream LSv neurons. Confirmation of AAV1-Cre-GFP expression in the PVH j and mCherry-expressing neurons in the LSv k and I. Scale bar = 100 µm. The white line enclosed areas in c–h outlining the PVH. Short arrows in k and l pointing to mCherry-expressing neurons. SCN: suprachiasmatic nucleus; LV: lateral ventricle; LSv: ventral part of lateral septum; fx: fornix; 3 V: the third ventricle; aca: anterior commissure area; AcbSh: accumbens shell

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