Fig. 2

Intraspecific variation in WRKY33 affects 4OH-ICN and immunity. a Schematic of WRKY33 proteins in Col-0, Col-0 wrky33, Ler-1, and Di-G. Black boxes denote WRKY domains (W), nuclear localization signal (NLS), or C-terminal domain (CTD). b LC-DAD-MS analysis of camalexin, ICN, and 4OH-ICN in seedlings inoculated with Psta for 24 h. Data represent mean ± SE of four replicates of 15 ± 2 seedlings each. c Bacterial growth analysis of Pst in surface-inoculated leaves. Middle and right panels were pre-treated with 20 μM dex for 6–8 h. Data represent mean ± SE of 4 (left); 11, 6, 7, 7, 7 (middle); 6, 6, 8 (right) replicates of 15 ± 2 seedlings each. CFU, colony-forming units. Different letters in b, c denote statistically significant differences (P < 0.05, one-factor ANOVA coupled to Tukey’s test). Source data of Figs. 2b and 2c are provided as a Source Data file