Fig. 4
Regulatory neofunctionalization of CYP82C2. a (Left) Phylogenetic species tree. (Right) HPLC-DAD analysis of 4OH-ICN in seedlings inoculated with Psta for 30 h. Data in box plots represent median (center line), 25th percentile (lower box limit), 75th percentile (upper box limit), and full range of variation (whiskers) for n = 13, 9, 3, 6, 6, 6, 6, 3, 3, 6 replicates of 15 ± 2 seedlings each. Data were pooled from several independent experiments with A. thaliana as the positive control. 4OH-ICA and 4OH-ICA-ME are aqueous and methanolic degradation products of 4OH-ICN, respectively. DW, dry weight; n.d., not detected. b (Left) Phylogenetic species tree. (Right) Synteny map of CYP82C genes. Gray arrows or rectangles represent non-CYP82C genes. Gray dotted lines represent large ( > 500 nt) sequence gaps. c, d qPCR analysis of 4OH-ICN and sideretin biosynthetic genes in seedlings inoculated with Psta (c) or grown in iron-deficient medium (d). Data in c represent mean ± SE of 3 (12 h Arabidopsis lyrata) or 4 (all other) replicates of 15 ± 2 seedlings each. Data in d represent mean ± SE of 3 replicates of 15 ± 2 seedlings each. Asterisks denote statistically significant differences of stress-treated relative to untreated samples (P < 0.05, two-tailed t-test). Source data of Figs. 4a, 4c, and 4d are provided as a Source Data file
