Fig. 1 | Nature Communications

Fig. 1

From: Engineered ribosomes with tethered subunits for expanding biological function

Fig. 1

Ribo-T system improvement strategies. a Schematic of Ribo-T showing tether (red) and orthogonal ribosome binding site (yellow). b The tether is optimized in cells growing exclusively from the Ribo-T plasmid. c Previously published Ribo-T tether sequence. d Orthogonal function evolved for Ribo-T. e Previously published orthogonal mRNA (o-mRNA) Shine-Dalgarno (SD) sequence and orthogonal 16S rRNA anti-SD (o-ASD) sequence shown

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