Fig. 5
From: Maternal control of suspensor programmed cell death via gibberellin signaling
Transient increase in, and polar distribution of, bioactive GA in seeds. a Left: Images of the nlsGPS1 emission ratios of unfertilized (UF), 4-DAP and 6-DAP seeds. Right: Images of the nlsGPS1 emission ratios of an optical section of a 4-DAP or 6-DAP seed. Boxes: micropylar endothecium. b Box plot of the nlsGPS1 emission ratios of the whole seed coat (n > 46 nuclei). c nlsGPS1 emission ratios of the micropylar and chalaza ends [dashed lines in a; n > 20 nuclei]. d Box plot of the nlsGPS1 emission ratios of stage 1 or 3 seeds [boxed region in a; n > 10 nuclei]. e NtCRF1-GFP was absent from the basal SC at stage 3. Fluorescence signal intensities along a line across all SCs (red arrows). X-axis, relative GFP signal intensity (green); y-axis, relative distances along the line. Gray shading, positions of nuclei. f and g Images of the nlsGPS1 emission ratios of 4-celled and 12-celled proembryos in WT background. Data are the means ± SD (standard deviations) (Student’s t-test; ns, P > 0.05; ***P < 0.001; ****P < 0.0001). ME micropylar end, CE chalaza end. Scale bars: 100 μm (a), 10 μm (e–g). The source data of the graphs are provided in the Source Data file
