Fig. 6
From: Maternal control of suspensor programmed cell death via gibberellin signaling
Ectopic expression of NtGA3ox1-GFP in the endothelium triggers PCD. a Localization of NtGA3ox1-GFP in the endothelium of pNtTPE8::NtGA3ox1-GFP plant. b Increased expression of NtGA3ox1 and decreased expression of NtCYS in the TG-8 and TG-11 lines. c TUNEL test in two-celled proembryos from WT and TG-8. d Frequency of two-celled proembryos with TUNEL-positive apical and basal cells in WT and TG-8 (n = 105–112). e Frequency of aborted seeds in WT and TG-8. (n = 1000–1500). f PI-staining of abnormal embryos with no suspensor in the TG-8 line. g NtCRF1-GFP signal in early proembryos after crossing WT and pNtCRF1::NtCRF1-GFP (CG) lines. Data are the means ± SE of 3 independent experiments in (b, c) and 5 in e (Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001); Scale bars: 150 μm (a), 10 μm (c, g), 20 μm (f). The source data of the graphs are provided in the Source Data file
