Fig. 5
From: Cytokinin functions as an asymmetric and anti-gravitropic signal in lateral roots
Signal peptide processing is required for CKX2 activity. a Localization of GFP-SPCKX2I-mScarlet and GFP-SPCKX2M-mScarlet in stage II LRs. Scale bar, 25 and 10 µm, respectively. b Quantification of the co-localization of GFP and mScarlet signal using Pearson’s correlation. Horizontal lines show the medians; box limits indicate the 25th and 75th percentiles; whiskers extend to the min and max values. Student’s t-test P-Value: ***P < 0.001, n = 10–12 individual LRs. c Immunoblot analysis and quantification of SPCKX2I and SPCKX2M expressed in N. benthamiana leaves using anti-GFP antibody. Anti-tubulin antibody was used as loading control. The signal of GFP-SP was quantified and normalized to tubulin. Student’s t-test P-Value: ***P < 0.001. Mean ± SEM, n = 4 biological replicates. d Saturation curves of isopentenyladenine (iP) degradation by CKX2. Reactions were performed at pH 7.4 in McIlvaine buffer with 0.5 mM DCIP as electron acceptor (black filled circle SPCKX2I, white square SPCKX2M, black filled triangle SPCKX2I, white diamond -SPCKX2M). Mean ± SEM, n = 8. e GSA distributions of ckx2-1 was complemented by pCKX2::CKX2I, but not by pCKX2::CKX2M. Representative lines are shown. Kolmogorov–Smirnov test P-value: ***P < 0.001 (compared to Col-0). Mean ± SEM, n = 5 plates (16 seedlings with 65–160 LRs per plate). a–e Experiments were repeated at least three times
