Fig. 4
From: The essential elements for the noncovalent association of two DNA ends during NHEJ synapsis
PAXX increases end proximity of two dsDNA but with modest efficiency. a Representative single-molecule time traces of donor (green) intensity, acceptor (magenta) intensity, and corresponding EFRET values (blue) for synapsis mediated by 25 nM Ku, 50 nM X4L4, and 50 nM PAXX. The right parts are histograms of donor intensity (green), acceptor intensity (magenta), and E values (blue) within the synapsis period. b Histogram of EFRET values of all synapsis events mediated by Ku, X4L4, and PAXX. The E value shown was obtained from a Gaussian fit of the highest peak of each kind of synaptic complex (FS and CS). n = 223 molecules. c Histogram of EFRET values of all synapsis events with EFRET ≥ 0.6. The E value was obtained from a Gaussian fit of the corresponding peak. n = 33 events. The data used here is the same batch as that used in b. d Dwell time of CS complex stimulated by PAXX alone or XLF plus PAXX. Only the high EFRET (E ≥ 0.6) events with both start and end time points within the detection time window were included. Error bars represent SD of two replicates. The corresponding dwell distributions and exponential fits are shown in Supplementary Figs. 6e, f. e Histogram of EFRET values of all synapsis events mediated by Ku, X4L4, XLF, and PAXX. The E value shown was obtained from a Gaussian fit of the highest peak of each type of synaptic complex (FS and CS). n = 429 molecules. f Fraction of low EFRET (FS) complex and high EFRET (CS) complex mediated by different combinations of NHEJ factors. Data are represented as the mean ± SD of at least three replicates. Source data are provided as a Source Data1 file
