Fig. 2

Secretion of CD63-GFP⁺ exosomes from CD63-GFP^f/+ primary neurons. a Representative size distribution of exosomes isolated from conditioned medium of cultured WT (green) and AAV-CaMKII-Cre transduced CD63-GFP^f/+ (red) primary neurons by the qNano particle analyzer. b Representative immuno-Electron Microscopy (EM) images of human and mouse CD63 labeling on in vitro prepared WT and CD63-GFP⁺neuronal exosomes. Scale bar: 100 nm; Subpanels i: anti-mouse CD63 on WT neuronal exosomes; ii: anti-mouse CD63 on CD63-GFP⁺ neuronal exosomes; iii: anti-human CD63 on CD63-GFP⁺ neuronal exosomes. Images were from a total of 40–50 images of two separate preparations. c Representative immunoblots of typical protein markers for exosomes, engineered exosome tags, and other subcellular organelles from lysate and purified exosome fractions of AAV-CaMKII-Cre transduced CD63-GFP^f/+ neurons. d Representative immunoblot of induced human CD63 and endogenous mouse CD63 from WT and CD63-GFP⁺ neuronal exosomes. Antibodies specifically recognizing either human or mouse CD63 were used