Fig. 5
Neuronal exosomes contain an abundant and selective subset of miRs. a Schematic diagram and volcano plot of differentially expressed neuronal and neuronal exosomal miRs identified by miR microarray hybridization. n = 3 biologically independent samples per condition. For each independent experiment, exosomes were prepared from same batch of primary neurons from which the total neuronal RNA was also prepared. N: neurons; E: neuronal exosomes; miRs with expression values < 23 arbitrary unit (A.U.) from the microarray hybridization are deemed not expressed. b Identification of enriched or selective miRs contained in either neurons or secreted exosomes by miR microarray hybridization. Green shaded area: miRs not expressed in either neurons or exosomes ( < 23 A.U. in both E and N); red shaded area: miRs selectively expressed in neurons ( < 23 A.U. in E, > 23 A.U. in N, FDR q < 0.05); purple shaded area: miRs selectively expressed in exosomes ( < 23 A.U. in N, > 23 A.U. in E, FDR q < 0.05); red dots: miRs enriched in E (FCE/N > 4, P < 0.05); blue dots: miRs enriched in N (FCE/N < -4, FDR q < 0.05); black dots: miRs not enriched in either N or E (FC < 4, FDR q < 0.05). c Representative selective or enriched miRs from both neurons and neuronal exosomes, respectively. N.D.: not detected; N/A: not applicable; dValidation of representative miRs that are neuron and exosome selective or enriched, respectively, by qPCR. All original Ct values are within 26–32 range, Ct > 34 is deemed not detected (N.D.). U6 small nuclear (sn) RNA was used as the endogenous control. n = 3–6 biologically independent samples (four technical replicates/sample) per condition. P-values were determined from two-tailed unpaired t-test. Error bars were presented in S.E.M
