Fig. 3
From: Supramolecular trap for catching polyamines in cells as an anti-tumor strategy
Confirmation of cell apoptosis. Contour diagram of Annexin V-FITC/PI flow cytometry results of MCF-7 cells incubated for 48 h. a Cells treated with PBS were used as a negative control; b cells treated with 25 μM P1P5A; c cells treated with 50 μM P1P5A. TUNEL analysis of MCF-7 cells incubated for 48 h. The apoptotic cells were labeled with FITC, while the nuclei were stained with DAPI; d cells treated with PBS were used as a negative control; e cells treated with 25 μM P1P5A; f cells treated with 50 μM P1P5A (scale bar: 24 μm). TEM images of ultrathin sections of MCF-7 cells incubated for 48 h; g cells treated with PBS were used as a negative control; h the cells treated with 25 μM P1P5A showed condensed chromatin and disappeared organelles; i the cells treated with 50 μM P1P5A showed the formation of many blebs and swollen or hollow mitochondria
