Table 1 List of antibodies and assay-specific concentrations
From: A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier
Antibody | Company | Catalog # | Dilution |
|---|---|---|---|
CD24 | R&D | FAB5247A | 1:10 |
OB-cadherin | R&D | FAB17901G | 1:20 |
Podocalyxin | R&D | FAB1658P | 1:10 |
CD31 (AlexaFluor-647) | BD Pharmingen | 561654 | IF 1:50 |
VEGFR2 | Abcam | 2349 | IF 1.5:100 |
WGA (Rhodamine) | Vector | RL-1022 | IF 1:100 |
NPHS1 (FITC) | LifeSpan Biosciences | LS-C370063 | IF 1:100 |
Nephrin (NPHS1) | Invitrogen | PA5-20330 | WB: 1:1000 |
WT1 | Abcam | ab15249 | IF 1:50 |
Col IV α1,2 | Abcam | ab6311 | IF 1:50 |
Col IV α3 | Shigei Research Institute | H31 | WB: 1:100 |
Col IV α4 | Shigei Medical Research | RH42 | IF: 1:25 |
LAM α5 | Abbiotec | 251457 | IF 1:100 WB 1:500 |
PLA2R | Millipore Sigma | MABC942 | IF 1:25 |
PLA2R | LifeSpan Biosciences | LS-C153547 | WB: 1:500 |
IgG4 | LifeSpan Biosciences | LS-C351418-500 | IF: 1:20 |
IgG (FITC) | Abcam | ab97174 | IF 1:20 |
F-actin | Life Technologies | r37122 | 1 drop/ml |
B-actin | GeneTex | GTX109639 | WB: 1:1000 |
C3d | Abcam | ab17453 | WB: 1:1000 |
Heparan sulfate | Abcam | ab23418 | IF: 1:75 |
Syndecan-4 | ThermoFisher | 36–3100 | IF: 1:75 FC: 1:100 |
Syndecan-1 | Abcam | Ab34164 | IF: 1:75 FC: 1:100 |
EHD3 | Atlas Antibodies | HPA049986 | IF: 1:100 |
EHD3 | ThermoFisher | PA5-25963 | FC: 1:50 |
BAX | Santa Cruz Biotechnology | SC-493 | IF: 1:100 |
