Fig. 3

Allelic difference of functional variant, rs1978060 in Chr.22q11.21. a Reporter assays in MCF-7 cells. There was a significantly decreased transcriptional activity for the risk G-allele of rs1978060 compared to the non-risk A-allele. Error bars show standard deviation (S.D.) for each variant. Asterisks indicate statistically significant changes in paired comparison (t-test P < 0.01). n = 2 independent experiments. b Electrophoretic mobility shift assays with nuclear extracts from MCF-7 cells. There was specific bands for A-allele probe (lane 1, black arrow) and the G-allele probe (lane 2, white arrow) of rs1978060. Competition analyses were performed using an excess of the unlabeled A-allele probe (lane 3, lane 6) and G-allele probe (lane 4, lane 5) as competitors. c A super-shift assay using the FOXA2 antibody. The black arrow indicates a super-shifted FOXA2 complex in lane 3. Source data are provided as a Source Data file