Fig. 2
From: Protein determinants of dissemination and host specificity of metallo-β-lactamases
Expression of VIM-2 and SPM-1 in non-frequent hosts induces hypervesiculation and compromises fitness. a Relative fold OMV production in cultures of the indicated hosts (Ec, Pa, or Ab) expressing blaNDM-1, blaVIM-2, or blaSPM-1 at 20 µM of IPTG. OMVs were quantitated based on total protein concentrations, normalizing to OD600, and dividing by OD600-normalized OMV production in control samples (corresponding to cells harboring the empty vector). Data correspond to three independent experiments and are shown as the mean value. Error bars represent standard deviations (s.d). b Growth curves for E. coli, A. baumannii, and P. aeruginosa carrying the empty vector (Vector) or expressing blaNDM-1, blaVIM-2, or blaSPM-1 in aerobic conditions in LB medium at 37 °C with 20 µM of IPTG. OD600 of the cultures was recorded every hour for 15 h. Data correspond to three independent experiments and are shown as the mean value. Error bars represent standard deviations (s.d). Right top panel: bacterial viability in a spot plating assay. Overnight cultures from E. coli expressing the different MBLs were serially diluted and 10 µl aliquots spotted onto LB agar without (-) or with the addition of 50 µM of IPTG. Colonies were allowed to develop for 24 h at 37 °C. Source data are provided as a Source Data file
