Fig. 1

Time course of the specific antibody response of horses against the consensus short-chain α-neurotoxin (ScNtx). Horse hyperimmune sera raised against the ScNtx were titrated by enzyme-linked immunosorbent assay (ELISA). Dashed lines indicate samples used for neutralization tests, corresponding to days 98 (blue), 147 (red), and 205 (orange) of the immunization. Points represent mean ± SD of triplicate wells of the ELISA