Fig. 3

Temporal autocorrelation and variance analysis of nucleic envelope and plasma membrane fluctuations. a Original autocorrelation plot for cell bottom, nucleic envelope, and plasma membrane at a single spatial position of the corresponding interface. b Dot plot of variance of membrane fluctuations measured at multiple spatial locations based on the autocorrelation plot shown in a, which is the correlation value obtained at the 0-time point. The line within each box represents the median, and the lower and upper boundaries of the box indicate the first and third quartiles, respectively. Error bars (whiskers) represent the interquartile range (n = 10). c Extrapolated autocorrelation plots, where white noise at the zero-time point has been corrected. d Variance of membrane fluctuations measured at multiple spatial locations based on the extrapolated autocorrelation plots c. The line within each box represents the median, and the lower and upper boundaries of the box indicate the first and third quartiles, respectively. Error bars (whiskers) represent the interquartile range (n = 10)