Fig. 2 | Nature Communications

Fig. 2

From: CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis

Fig. 2

CD36 plays anti-carcinogenic roles via repressing glycolysis. a CCK8 assays and colony formation assays, transfection efficiency was determined by western blot. b Cell apoptosis under normal conditions and 5-Fu treatment and western blots of apoptosis markers. c Cell cycle analysis and western blots of cell cycle markers. d Gene-set enrichment analysis (GSEA) of the protein profiles between SW480 LV-CD36 and SW480 LV-RFP cell lines. e 2-NBDG uptake, lactate production and ATP production. f Cell viability was measured by MTT assays (up) under different culture conditions. Cell apoptosis of SW480 and LoVo cells (LV-RFP vs. LV-CD36) with low glucose conditions (down). Each experiment was performed at least triplicate and results are presented as mean ± SEM. Student’s t-test, one-way ANOVA or two-way ANOVA was used to analyze the data (*P < .05, **P < .01, ***P < .001, ****P < .0001). Source data are provided as a Source Data file

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