Fig. 7
AAV-mediated CD36 knockdown promotes CRC. a Macroscopic appearance of tumors in the large intestines of AOM-DSS-induced mice. Intestinal tumor numbers and tumor volumes were measured. b Representative images of IHC staining of indicated targets on tumor sections. Scale bar, 20 μm (40×). c Macroscopic appearance of tumors in the large intestine of ApcMin/+ mice, statistical analysis of tumor numbers and sizes in the colon and rectum. d Representative IHC staining of PCNA, cell proliferation index was calculated as before. All statistical results are shown as mean ± SEM, based on Student’s t-test, *P < .05, **P < .01, ***P < .001. e Representative images of IHC staining of CD36, GPC4, β-catenin, c-myc, and downstream glycolytic genes GLUT1, HK2, PKM2 and LDHA on the tumor sections. Scale bar, 20 μm (40×). f Schematic diagram summarizing our working model, namely, CD36 can interact with and induce the proteasome-dependent ubiquitination of GPC4, thereby inhibiting β-catenin/c-myc signaling, followed by repressed glycolytic activity and colorectal tumor suppression. Source data are provided as a Source Data file
