Fig. 6
Bu and Cy trigger key signaling pathways for proliferation and cell death. a Schedule for Bu and/or Cy treatment and subsequent protein analysis after 1, 3, 6, and 12 h of treatment. b Protein analysis after Bu and/or Cy treatment comparing the concentrations of phosphorylated PI3K, phosphorylated and total Akt, cyclin D1, p53, phosphorylated and total p38, and p21, with β-actin as a loading control (n = 6 biological samples, Supplementary Fig. 7). c Schedule for sequential Bu/Cy treatment and subsequent protein analysis after 0, 1, 3, 6, 16, 18, 21, and 27 h of treatment. d Protein analysis after sequential Bu/Cy treatment comparing the concentrations of phosphorylated PI3K, phosphorylated and total Akt, cyclin D1, p53, phosphorylated and total p38, and p21, with β-actin as a loading control (n = 4 biological samples; Western blotting). Bu busulfan, Cy cyclophosphamide, Bu/Cy busulfan followed by cyclophosphamide
