Fig. 1 | Nature Communications

Fig. 1

From: Single cell transcriptome analysis of developing arcuate nucleus neurons uncovers their key developmental regulators

Fig. 1

scRNA analysis of E15 ARC. a Schematics for dissecting out the developing ARC region from E15 Pomc-eGfp embryos. b Spectral tSNE plot of 5038 cells from E15 ARC, colored according to gene expression, reveal 12 clusters of cells (the clusters c0 to c11). The number of cells (#) in each cluster and the most specific gene in each cluster are as shown. c Spectral tSNE plot of 5038 cells from E15 ARC, colored for the expression of representative key marker genes of ARC neurons, Npy/Sst/Otp for AgrpSst-neurons, Ghrh/Dlx1 for Ghrh-neurons, Dlx1/Th for Th-neurons, Pomc/Nhlh2 for POMC-neurons, and Sox14/Nhlh2 for Kisspeptin-neurons. d Overlap of three spectral tSNE plots of 5038 cells from E15 ARC, colored differently for Npy (blue), Ghrh (red) and Sox14 (green), reveals that their expressions in the cluster c0 are mutually exclusive, suggesting that the cluster c0 likely represents a pool of multiple ARC neuronal types

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