Fig. 5
Crucial roles of Foxp2 in the development of Ghrh-neurons. a IHC with antibodies against Foxp2 and either Dlx1 or Gsx1 reveals co-expression of Foxp2 and Dlx1/Gsx1 in a subset of E15.5/E17.5 ARC neurons. b ISH for Ghrh (blue) combined with IHC against Foxp2 (brown) reveals co-expression of Foxp2 with Ghrh in P0 ARC. c ISH against Ghrh reveals that the number of Ghrh+ cells (i.e., Ghrh-neurons) in the ARC is significantly reduced in Foxp2-cKO mice relative to control mice at both E17.5 and P100 (in both sexes). The number of mice per each genotype was 3–5. The distance between serial sections was 132 μm, and the images shown are for the second posterior sections. d Dwarfism of P50 Foxp2-cKO male and female mice, accompanied by significant reductions in body weight (BW), linear growth (length, cm), and the relative expression levels of IGF1 in the liver. The number of mice for each genotype was 3–5. All scale bars are for 100 μm. Statistical differences were determined by Student’s t-test (c, d); p < 0.05 (one asterisk) and p < 0.01 (two asterisks)
