Fig. 5 | Nature Communications

Fig. 5

From: An anaerobic bacterium host system for heterologous expression of natural product biosynthetic gene clusters

Fig. 5

Activation of BGC4 and metabolic profiles of S. mutans UA159*/ldhP-BGC4. a Two constitutive promoters gyrAp and ldhP were inserted to replace the transcriptional regulatory gene orfD in S. mutans UA159*/BGC4 to obtain S. mutans UA159*/ldhP-BGC4. Functional NRPS and PKS domains are indicated in bold: A, adenylation domain; C, condensation domain; Cs, starter condensation domain; Cy, heterocyclization domain; Ox, oxidative domain; AT, acyl-transferase; KS, ketosynthase; T, thiolation domain; TE, thioesterase. b HPLC traces of the supernatant extracts of S. mutans UA159*/ldhP-BGC4, S. mutans UA159*-RS/BGC4 and S. mutans UA159*/ldhP-BGC4 ∆orfF were used as negative controls. c Structures of SNC1-465 and its analogue compound 1

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