Fig. 7
From: MICU1 controls cristae junction and spatially anchors mitochondrial Ca2+ uniporter complex
EMRE redistributes in a MCU-dependent manner to the IBM under ER Ca2+ release. a Representative SIM images of HeLa cells that were transiently transfected with EMRE-mCherry (magenta) and MICU1-YFP (green) under resting conditions and 90 s after stimulation with 100 µM histamine. b The sub-mitochondrial localization of EMRE-mCherry under resting conditions (−Hist) and 90 s after treatment with histamine (100 µM) (+Hist) was determined by the IBM association index (n = 8). c Identical experiments as those in a but using cells that overexpressed wild-type MCU. d Identical experiments as those in b but using cells that overexpressed wild-type MCU (n = 8). Horizontal lines represent the median, the lower and upper hinge show respectively first quartile and third quartile, and lower and upper whisker encompass minimal and maximal values. Images and analyses were obtained from at least 10 cells in each of 8 experiments. *P < 0.05 vs. respective control conditions carried out with unpaired double-sided T-test. Source data are provided as a Source Data file
