Fig. 2
From: An electrostatic switching mechanism to control the lipid transfer activity of Osh6p
Osh6p mutants extract differently PS and PI4P. a PS extraction assay. Fluorescence spectra of NBD-C2Lact (250 nM) measured upon excitation at 460 nm in the presence of liposomes (80 µM, 2% POPS) before (black spectrum) and after (pink spectrum) adding Osh6p or mutants (3 µM). A reference spectrum (in gray) was recorded with pure DOPC liposomes incubated with NBD-C2Lact. b Percentage of accessible PS extracted by each construct based on signal normalization at 536 nm. (n = 5–6, **P < 0.01, Mann–Whitney test). c PI4P extraction assay. Fluorescence spectra of NBD-PHFAPP (250 nM) (λex = 460 nm) mixed with liposomes (80 µM, 2% diC16:0-PI4P) before (black spectrum) and after (orange spectrum) adding 3 µM Osh6p WT or mutants. A reference spectrum of NBD-PHFAPP (in grey) was recorded with PI4P-free liposomes. d Percentage of accessible PI4P extracted by each construct based on signal normalization at 536 nm (n = 4,*P < 0.05, Mann–Whitney test). Error bars correspond to s.e.m. Source data are provided as a Source Data file
