Fig. 2

Omission cue-induced suppression (OCIS) procedure for alcohol seeking. All data are mean and SEM. Gray open circles depict individual data-points. Thirty-three rats were initially used and randomly assigned to alcohol and stress priming groups (n = 15,18). Of which, 25 rats were retained for statistical analyses (see Methods). Final Ns per group are described below. a Timeline and schedule. b Responses during the first 10-days of Self-administration (SA) training. c, d Responses during the last and first 10 days of Discrimination training under non-withdrawal (NW) and acute withdrawal (AW) conditions. Operant training was resumed 4–5 days following the diet schedule change to induce AW. n = 25. Two-way repeated measures ANOVA: Withdrawal (F(1,24) = 18.27, P < 0.001) main effects. #P < 0.01–0.05 vs. NW. Tuckey HSD test. e Responses during the first 1-h block of Discrimination test to determine the anti-relapse action of alcohol S- against alcohol availability cues under NW, AW, and protracted withdrawal (PW). n = 25. Two-way repeated measures ANOVA: Withdrawal (F(2,48) = 15.01, P < 0.001) and Cue-Test (F(1,24) = 120.78, P < 0.001) main effects, Withdrawal x Cue-Test interaction (F(2,48) = 11.85, P < 0.001). #P < 0.01–0.05 vs. NW. *P < 0.001 vs. No S-. Tuckey HSD test. Duplicate data-points from multiple subjects are not overlaid on this Figure for clarity because n = 25. Thus, the dot plots represent the data range. f Responses during the second and third 1-h blocks of Discrimination test to determine alcohol S-‘s action against alcohol priming under NW, AW, and PW. n = 12. Three-way repeated measures ANOVA: Withdrawal (F(2,22) = 13.22, P < 0.001) and Priming (F(1,11) = 97.50, P < 0.001) main effects, and Withdrawal x Priming (F(2,22) = 16.48, P < 0.001), Cue-Test x Priming (F(1,11) = 24.22, P < 0.001) and Withdrawal x Cue-Test x Priming (F(2,22) = 14.78, P < 0.001) interactions. #P < 0.01–0.05 vs. NW. †P < 0.01 vs. water. *P < 0.01–0.05 vs. No S-. Bonferroni test. g Responses during the second and third 1-h blocks of Discrimination test to determine alcohol S-‘s action against stress priming under NW, AW, and PW. n = 13. Three-way repeated measures ANOVA: Priming (F(1,12) = 27.65, P < 0.001), Withdrawal x Priming (F(2,24) = 8.34, P < 0.01), Cue-Test x Priming (F(1,12) = 17.70, P < 0.01), and Withdrawal x Cue-Test x Priming (F(2,24) = 6.20, P < 0.01) interactions. #P < 0.05 vs. NW. †P < 0.01 vs. saline. *P < 0.05 vs. No S-. Bonferroni test