Fig. 2

RST1 suppresses silencing of transgenes. a The rst1-4 mutation suppresses the developmental phenotype induced by a MIM156 transgene. b Diagram of the AT3G27670 gene encoding the RST1 protein. Boxes represent exons, lines represent introns. Triangles indicate the position of the T-DNA insertions in rst1-2 and rst1-3 lines. Vertical lines indicate the point mutations in rst1-4 and rst1-5. c rst1-4 is a weak allele. Accumulation of the CER3 mRNA (left) and CER3-derived siRNAs (right) in wild-type (WT), cer7-3 and the four rst1 alleles used in this study shown by RNA blots hybridised with a probe specific to CER3. The methylene blue stain of the membrane (MB) and hybridisation to U6 snRNA are shown as loading controls. d RNA blots showing the accumulation of the full-length MIM156 ncRNA and MIM156-derived siRNAs visualised by hybridisation with a probe specific to the IPS1 backbone of the MIM156 transgene. 7SL RNA and U6 snRNA are shown as loading controls. e RNA blots showing that the rst1-5 mutation results in reduced levels of the GUS mRNA and increased levels of GUS-derived siRNAs in the L1 jmj14-4 background. 25S rRNA and U6 snRNA are shown as loading controls. f The rst1-5 mutation increases S-PTGS frequency in both 6b4 and Hc1 reporter lines. The barplot shows the proportion of plants with silenced GUS expression in the indicated genotypes. The source data are available at [https://doi.org/10.6084/m9.figshare.c.4483406]