Fig. 5

Molecular signatures associated with each metabolic phenotype. a Copy-number plot across the chromosomes for 2-NBDG^high (green) and C12R^high (blue) cells for three patient MPE samples, highlighting their genome-profile similarity between the two metabolic phenotypes. Blue indicates deletion and red amplification. b Venn Diagram of the differentially expressed genes (DEGs) between two metabolic phenotypes across five patient MPE samples. A total of 179 DEGs are shared among five patients. c Log2 fold change of gene expression levels between the two metabolic phenotypes (2-NBDG^high vs C12R^high) across five patients. Representative genes involved in glycolysis, mitochondrial ETC complexes, and fatty acid oxidation are listed. d Enrichment of the DEGs up-regulated in each metabolic phenotype and shared by at least 4 out of 5 patients against 5 representative public databases by Enrichr. The top two entries ranked by the combined scores from each database are plotted (see also Supplementary Data 5 for p values and enrichment scores). e Gene set enrichment analysis of 5 relevant pathways across the five patients (P10, P12, P14, P17, and P24 from bottom to top respectively). NES denotes normalized enrichment score (*P < 0.05 and FDR q < 0.25). f Log2 fold change of gene expression levels between the two metabolic phenotypes (2-NBDG^high vs C12R^high) across five patients. Representative epithelial and mesenchymal markers as well as immune checkpoint ligands and MHC Class I-associated HLA genes are listed