Fig. 1
Immunogenicity of SFTSV DNA vaccines in BALB/c mice. a Diagram of SFTSV DNA vaccines comprising five plasmids encoding SFTSV antigens derived from the common sequence of 31 SFTSV strains. b Western blot analysis of SFTSV DNA vaccine expression in 293T cells. c Immunization schedule (Supplementary Fig. 1a shows a detailed timeline of vaccination and immune analysis). d T-cell immunogenicity of SFTSV DNA vaccines in BALB/c mice. IFN-γ ELISpot assays were performed to detect antigen-specific IFN-γ-producing T cells by stimulating splenocytes from immunized mice with overlapping peptide (OLP) pools. Data (mean ± s.e.m.) represent the average number of spot-forming units (SFUs) per 5 × 105 splenocytes. e Frequency of effector memory (CD44+CD62L−) CD8+ T cells among SFTSV NSs247–255-specific CD8+ T cells. Splenocytes from vaccinated mice were stained with H-2Ld NSs247–255 dextramer to identify SFTSV NSs247–255-specific CD8+ T cells. Then the frequency of CD44+CD62L−CD8+ T cells in this population was examined by multi-color FACS analysis. f Neutralizing antibody response to SFTSV CB1/2014 strain (genotype B) generated by SFTSV DNA vaccines in BALB/c mice. In anti-sera collected from vaccinated mice, the amount of neutralizing antibody against SFTSV was determined based on FRNT50. Error bars indicate the mean ± s.e.m. Statistical significance was determined by one-way ANOVA test with Tukey correction. **p < 0.01; ***p < 0.001; ****p < 0.0001. Source data are provided as a Source Data file
