Fig. 3

Maintenance of lung tissue CD64⁺ mononuclear phagocytes in adult C57BL/6 mice. a Experimental outline for panels (b, c). Briefly, at 4 weeks of age, Cx3cr1^CreERT2.Rosa26-LSL-YFP mice were treated with TAM s.c. 3 times, 48h apart. Mice were analyzed for YFP expression 2, 9, 28, and 52 weeks later. b Representative histograms of YFP expression within the indicated populations. Numbers indicate the percentage of YFP⁺ cells, as quantified in (c). c Percentage of YFP⁺ cells within the indicated populations, assessed by flow cytometry. d Percentages of Ki-67⁺ cells in the indicated populations. e, f Absolute numbers of the indicated cell populations in the lungs of e Ccr2^−/− or f Nr4a1^−/− and control WT mice. c–f Data show mean ± s.e.m., as well as individual mice in (d–f) (c, n = 10; d, n = 4; e, f, n = 9–12) and are pooled from 2 to 3 independent experiments. P-values were calculated using (c) a two-way ANOVA with Tukey’s post hoc test, d non-parametric Mann–Whitney test for pairwise comparisons or e, f a two-tailed unpaired Student’s t-test. °/*P < 0.05; **P < 10⁻²; ***P < 10⁻³; ****P < 10⁻⁴; ns, not significant. In c the empty circle compares CD206⁺ and CD206⁻IM at week 52. Source data are provided as a Source Data file. s.c., subcutaneous; TAM, tamoxifen