Fig. 4

Localization of lung CD64⁺CD16.2⁺ monocytes, CD206⁺ IM and CD206⁻ IM a Confocal microscopy pictures of lung sections from Cx3cr1^GFP/GFP mice (CX3CR1 [green]; CD68 [red]; DAPI [blue]; CD16.2, MHC-II or CD206 [white]). CD64⁺CD16.2⁺ monocytes, CD206⁺ IM and CD206⁻ IM were identified as CX3CR1⁺CD68⁺CD16.2⁺, CX3CR1⁺CD68⁺CD206⁺ and CX3CR1⁺CD68⁺MHC-II⁺cells, respectively. Asterisks indicate CX3CR1⁻CD68⁺ AM; plain or empty arrows indicate CX3CR1⁺CD68⁺ cells expressing or not the marker of interest (i.e., CD16.2, CD206 or MHC-II), respectively. b Preferential distribution of the indicated populations in the peribronchial/perivascular area vs. the alveolar parenchyma. c, d Lung sections of C57BL/6 WT mice were analyzed: CD68 [red]; c CD31 or d Tubb3 [white]; DAPI [blue]; MHC-II, CD206 or CD16.2 [green]). CD206⁺ IM, CD206⁻ IM and CD64⁺CD16.2⁺ monocytes were identified as CD68⁺CD206⁺, CD68⁺MHC-II⁺ and CD68⁺CD16.2⁺ cells, respectively. b Data show mean ± s.e.m. and are pooled from 2 independent batches of mice (n = 4–6). P-values were calculated using a Kruskal–Wallis test, and pairwise comparisons were estimated using Mann–Whitney tests. **P < 10⁻²; ns, not significant. Source data are provided as a Source Data file. Scale bars = (a) 100 µm; (c,d) 50 µm