Fig. 1
From: Propagation of temporal and rate signals in cultured multilayer networks
Stimulation of neurons in cultured multilayer networks. a Neurons, visualized with IR-DIC and fluorescent microscopy, are grown in distinct layers (0.7 × 6 mm2) separated by 0.4 mm. b Schematic of the multilayer network. Cell-attached or whole-cell recordings could be performed from four neurons in sequential layers. c Using a Digital Light Processing (DLP) projector mounted on a microscope, brief light pulses (blue boxes in a) were delivered to neurons (in layer 1) that expressed ChR2 and a fluorescent tag (green)
