Fig. 9
From: Propagation of temporal and rate signals in cultured multilayer networks
Propagation in networks of different sizes. a Schematic of the multilayer network with compartments of different sizes. All layers were 0.5 mm in width with lengths of 0.5, 1, 1.5, and 2 mm from top to bottom. An area of same dimension was photostimulated either in the smallest (magenta) or in the largest (cyan) compartment. b, c Average poststimulus time histogram of neurons when the small (b) or the large (c) network was stimulated. In each network, a single neuron is recorded in each layer (from black to magenta (cyan) for layers 1–4, respectively). Data were averaged from n = 11 networks of density = 297 ± 111 neurons·mm−2 (mean ± STD). d–f Spike probability (d), firing rate (e), and delay of first spike (f) as a function of layer when the smallest (magenta) or the largest (blue) network was activated. In d–f all data are shown and the mean ± SEM is represented as thick lines. Paired Mann–Whitney U-test statistics: d L1: P = 1, L2: P = 0.643, L3: P = 0.024, L4: P = 0.019; e L1: P = 0.898, L2: P = 0.898, L3: P = 0.102, L4: P = 0.067; f L1: P = 0.966, L2: P = 0.465, L3: P = 0.278
