Fig. 5
From: SOX11 and SOX4 drive the reactivation of an embryonic gene program during murine wound repair
Identifying downstream effectors of SOX11 and SOX4 in embryonic epidermis. a qRT-PCR analysis of isolated epidermis from Sox11 cKO, Sox4 cKO, or dcKO and their WT littermate embryos at E16.5 with two biological replicates per genotype. The values are gene expression levels in the knockouts relative to their respective wild-type controls. Data are the mean ± SD. n = 2 biological replicates. b Two-color microarray analysis was performed on E16.5 epidermis lacking either Sox11 or Sox4, or both genes, as well as on wild-type littermate, which is used as a baseline control. Heat map shows clustering of probesets with significant differential expression (FDR < 0.05 and fold change > 1.5) in dcKO, Sox11 cKO or Sox4 cKO epidermis relative to their WT littermate controls. n = 2 biological replicates. c Venn diagram showing the overlap of probesets significantly changed in dcKO, Sox11 cKO or Sox4 cKO epidermis. d Cell organization/motility (blue) and epidermal development (red) are among the top GO biological processes found in the differentially expressed genes in dcKO epidermis at E16.5 (as determined by Metascape, q values < 0.05). e Venn diagrams (Top panel) show the overlaps of genes up- or downregulated (log2-fold change ≥ 1.5 and FDR < 0.05) in E16.5 dcKO epidermis with genes up- or downregulated in E13.5 epidermal cells as compared to P4 basal epidermal cells. Hypergeometric p values and the enrichment level (R) of the overlap show statistical significance between specified groups (highlighted in red). f Graph shows the percentage of genes altered in dcKO overlapping with embryonic signature genes. Source data are provided as a Source Data file
