Fig. 6 | Nature Communications

Fig. 6

From: YAP1 subgroup supratentorial ependymoma requires TEAD and nuclear factor I-mediated transcriptional programmes for tumorigenesis

Fig. 6

The YAP1-MAMLD1 exerts its oncogenicity via TEAD-mediated transcriptional control. a Western blot of human CTGF and CYR61 in LN229 cells transiently transfected with indicated genes. The transgene protein products were tagged by HA. b Western blot of pan-TEAD proteins (pan-TEADs) after immunoprecipitation with an anti-HA antibody for HA-tagged YAP1-MAMLD1 fusion protein, confirming that the S94A-mutated YAP1-MAMLD1 fusion has lost the ability to bind TEAD transcription factors. c Western blot of YAP1-Tead targets CTGF and CYR61 using NIH/3T3 cells transfected with indicated genes. The transgene protein products were tagged by HA. d, e Luciferase-based d in vivo bioluminescence images at weeks 1–3 after birth and e Kaplan–Meier curves of animals electroporated with S94A-mutated YAP1-MAMLD1 fusion. Cyan curve is same as in Fig. 3k

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