Fig. 2 | Nature Communications

Fig. 2

From: Emulating evolutionary processes to morph aureothin-type modular polyketide synthases and associated oxygenases

Fig. 2

Phylogenetic analysis of the aur PKS and nor PKS. a The aur-type and nor-type gene clusters. The original producers of aureothin and neoaureothin are underlined. b The HPLC profile of authentic reference of neoaureothin (2, middle panel), and S. scabrisporus culture (bottom panel). HR-MS spectrum of neoaureothin in top panel. UV detection is at 350 nm. c Phylogenetic tree of KS domain amino acid sequences of the aur-type and nor-type PKSs and other actinobacterial PKSs. The tree was reconstructed by Bayesian inference. Numbers at nodes indicate clade credibility values. Branch length represents the number of amino acid changes per position. Olm, oligomycin; Tyl, tylacton; Nan, nanchangmycin; Mon, monensin; Fsc, candicidin; Pim, pimaricin; Nys, nystatin; Amp, amphotericin; Ave, avermectin; Pte, polyene macrolide from S. avermitilis. Color code for KS clades corresponds with those shown in Fig. 1 d. Possible evolutionary scenarios between aur PKS and nor PKS. The source data underlying Fig. 2c are provided as a Source Data file

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