Fig. 5

Overview and metabolic profile of modified aur PKS variants nor^# PKS and nor* PKS. a Domain architectures of the nor^# PKS and nor* PKS. The N-terminal docking domain of aurB is swapped to that of norB in the nor^# PKS. In the nor* PKS, the length of recombinant KS-AT linker is manipulated to match that of the nor PKS. b The fusion sites are within the KS-AT linker and induced by the restriction enzyme HpaI and SpeI. The amino acid sequences at the fusion regions of nor PKS and nor* PKS are aligned. c HPLC profile of authentic standard of aureothin (1), neoaureothin (2), deoxyneoaureothin (6), and S. albus mutant strains. UV detection is at 350 nm